Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells

Kaur, R; Chen, J; Dawoodji, A; Cerundolo, V; Garcia-Diaz, Y; Wojno, J; Cox, L; Besra, G; Moghaddam, B; Perrie, Y

Journal article

Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells

Abstract:: Dendritic cells (DCs) are able to present glycolipids to invariant natural killer T (iNKT) cells in vivo. Very few compounds have been found to stimulate iNKT cells, and of these, the best characterised is the glycolipid α-galactosylceramide, which stimulates the production of large quantities of interferon-gamma (IFN-γ) and interleukin-4 (IL-4). However, αGalCer leads to overstimulation of iNKT cells. It has been demonstrated that the αGalCer analogue, threitol ceramide (ThrCer 2), successfully activates iNKT cells and overcomes the problematic iNKT cell activation-induced anergy. In this study, ThrCer 2 has been inserted into the bilayers of liposomes composed of a neutral lipid, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), or dimethyldioctadecylammonium bromide (DDA), a cationic lipid. Incorporation efficiencies of ThrCer within the liposomes was 96% for DSPC liposomes and 80% for DDA liposomes, with the vesicle size (large multilamellar vs. small unilamellar vesicles) making no significant difference. Langmuir-Blodgett studies suggest that both DSPC and DDA stack within the monolayer co-operatively with the ThrCer molecules with no condensing effect. In terms of cellular responses, IFN-γ secretion was higher for cells treated with small DDA liposomes compared with the other liposome formulations, suggesting that ThrCer encapsulation in this liposome formulation resulted in a higher uptake by DCs. © 2011 Wiley-Liss, Inc. and the American Pharmacists Association.

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APA Style

Kaur, R., Chen, J., Dawoodji, A., Cerundolo, V., Garcia-Diaz, Y., Wojno, J., Cox, L., Besra, G., Moghaddam, B., & Perrie, Y. (2011). Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells. Journal of Pharmaceutical Sciences, 100(7), 2724–2733.

MLA Style

Kaur, R., et al. “Preparation, Characterisation and Entrapment of a Non-Glycosidic Threitol Ceramide into Liposomes for Presentation to Invariant Natural Killer T Cells.” Journal of Pharmaceutical Sciences, vol. 100, no. 7, 2011, pp. 2724–33.

Chicago Style

Kaur, R, J Chen, A Dawoodji, V Cerundolo, Y Garcia-Diaz, J Wojno, L Cox, G Besra, B Moghaddam, and Y Perrie. 2011. “Preparation, Characterisation and Entrapment of a Non-Glycosidic Threitol Ceramide into Liposomes for Presentation to Invariant Natural Killer T Cells.” Journal of Pharmaceutical Sciences 100 (7): 2724–33.
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